Orientia tsutsugamushi
Product name
HWTS-OT002B Orientia tsutsugamushi Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Scrub typhus is an acute febrile disease caused by Orientia tsutsugamushi (Ot) infection. Orientia scrub typhus is a Gram-negative obligate intracellular parasitic microorganism. Orientia scrub typhus belongs to the genus Orientia in the order Rickettsiales, family Rickettsiaceae, and genus Orientia. Scrub typhus is mainly transmitted through the bites of chigger larvae carrying pathogens. It is clinically characterized by sudden high fever, eschar, lymphadenopathy, hepatosplenomegaly, and peripheral blood leukopenia, etc. In severe cases, it can cause meningitis, liver and kidney failure, systemic multi-organ failure, and even death.
Channel
| FAM | Orientia tsutsugamushi |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | fresh serum |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copies/μL |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Recommended extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be conducted according to the instruction for use of this extraction reagent. The extracted sample volume is 200µL, and the recommended elution volume is 100µL.
