The kit is used for the in vitro qualitative detection of 14 types of human papillomaviruses (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) specific nucleic acid fragments in human urine samples, female cervical swab samples, and female vaginal swab samples, as well as HPV 16/18/52 typing, to aid in the diagnosis and treatment of HPV infection.