professional factory for Ev71 And Coxa16 Nucleic Acid Detection Kit (Fluorescence Pcr) - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test
professional factory for Ev71 And Coxa16 Nucleic Acid Detection Kit (Fluorescence Pcr) - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test Detail:
Product name
Macro & Micro-Test Sample Release Reagent
Certificate
CE, FDA, NMPA
Features
Rapid: only 5 mins.
Easy: only 3 steps (1.add the sample 2.Mix upside and down 10 times 3. Leave it for 5 mins).
Main components
| Name | Main components | Component specifications | Quantity |
| Sample Release reagent | Dithiothreitol, sodium dodecyl sulfate (SDS), RNase inhibitor, surfactant, purified water | 0.5mL/Vial | 50 Vial |
Note: Components in different batches of kits are not interchangeable.
Storage conditions and shelf life
Store and transport at room temperature. The shelf life is 24 months.
Applicable instruments
Instruments and equipment during sample processing, such as pipettes, vortex mixers, water baths, etc.
Sample requirements
Freshly collected oropharyngeal swabs, nasopharyngeal swabs.
Precision
When this kit is used for extraction from the in-house precision reference CV for 10 replicates, the coefficient of variation (CV, %) of Ct value is not more than 10%.
Inter-batch difference
When the in-house precision reference is tested on three batches of kits under trial production upon repeated extraction and, the coefficient of variation (CV, %) of Ct value is not more than 10%.
Performance comparison
● Extraction efficiency comparsion
|
Efficiency comparsion of magnetic beads method and sample releaser |
||||
|
concentration |
magnetic beads method |
sample releaser |
||
|
orfab |
N |
orfab |
N |
|
|
20000 |
28.01 |
28.76 |
28.6 |
29.15 |
|
2000 |
31.53 |
31.9 |
32.35 |
32.37 |
|
500 |
33.8 |
34 |
35.25 |
35.9 |
|
200 |
35.25 |
35.9 |
35.83 |
35.96 |
|
100 |
36.99 |
37.7 |
38.13 |
undet |
The extraction efficiency of sample releaser was similar to that of magnetic beads method, and the concentration of pathogen could be 200Copies/mL.
● CV value comparison
|
Repeatability of sample releaser extraction |
||
| concentration:5000Copies/mL |
ORF1ab |
N |
|
30.17 |
30.38 |
|
|
30.09 |
30.36 |
|
|
30.36 |
30.26 |
|
|
30.03 |
30.48 |
|
|
30.14 |
30.45 |
|
|
30.31 |
30.16 |
|
|
30.38 |
30.7 |
|
|
30.72 |
30.79 |
|
| CV |
0.73% |
0.69% |
When tested at 5,000 copies /mL, the CV of orFab and N was 0.73% and 0.69%, respectively.
Product detail pictures:
Related Product Guide:
We purpose to understand high quality disfigurement with the output and supply the top service to domestic and overseas buyers wholeheartedly for professional factory for Ev71 And Coxa16 Nucleic Acid Detection Kit (Fluorescence Pcr) - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test , The product will supply to all over the world, such as: Mauritania, Nepal, Tajikistan, We are looking forward to establishing a mutually beneficial relationship with you based on our high-quality products, reasonable prices and best service. We hope that our products will bring you a pleasant experience and carry a feeling of beauty.
By Freda from Swiss - 2018.11.04 10:32
With a positive attitude of regard the market, regard the custom, regard the science, the company works actively to do research and development. Hope we have a future business relationships and achieving mutual success.
By Queena from Bolivia - 2018.11.06 10:04
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