Quality Inspection for Mycobacterium Tuberculosis Test - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Quality Inspection for Mycobacterium Tuberculosis Test - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)
Freeze-dried Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)

Certificate

CE

Epidemiology

Mycobacterium culosis is referred to as Tubercle bacillus(TB). Mycobacterium tuberculosis that is pathogenic to humans is now generally considered to be of human, bovine, and African types. Its pathogenicity may be related to the inflammation caused by the proliferation of bacteria in tissue cells, the toxicity of bacterial components and metabolites, and the immune damage to the bacterial components. Pathogenic substances are associated with capsules, lipids and proteins.

Mycobacterium tuberculosis can invade susceptible organisms through the respiratory tract, digestive tract or skin injury, causing tuberculosis of various tissues and organs, of which the most common is pulmonary tuberculosis through the respiratory tract. It usually occurs in children, and presents with symptoms such as low-grade fever, night sweats, and a small amount of hemoptysis. Secondary infection is mainly manifested as low-grade fever, night sweats, and hemoptysis. Mostly it is long-term chronic disease. In 2018, about 10 million people around the world were infected with Mycobacterium tuberculosis, of which about 1.6 million died.

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 100 bacteria/mL.
● High specificity: No cross-reactivity with the human genome and other non-Mycobacterium tuberculosis and pneumonia pathogens.
● Convenient: One test for multiple results.

Channel

FAM: Target (IS6110 and 38KD) nucleic acid DNA
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2mins No
2 1 cycle 95℃ 10mins No
3 40 cycles 95℃ 15s No
60℃ 40s Yes

Technical Parameters

Storage
Liquid ≤-18℃ In dark
Lyophilization ≤30℃
Shelf-life 12 months
Specimen Type Sputum
Ct ≤39
CV ≤5.0%
LoD 100 bacteria /mL

Specificity: No cross-reactivity with the human genome and other non-Mycobacterium tuberculosis and pneumonia pathogens.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
ABI 7500 Fast Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
BioRad CFX96 Real-Time PCR System, BioRad.
CFX Opus 96 Real-Time PCR System.

Main Components

Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)

Component

Catalogue Number

Quantity

Component Description

HWTS-RT001A HWTS-RT001B
Specification(50 tests/kit) Specification(20 tests/kit)
TB Reaction Buffer 1 mL/vial 400μL/vial 1 vial Mycobacterium tuberculosis IS6110 and 38KD, internal control specific primers, fluorescent probes, Taq enzyme, UDG  enzyme, reaction buffer, etc.
TB Positive Control 1 mL/vial 400μL/vial 1 vial IS6110, 38KD and internal control mixed plasmid diluent
TB Blank Control 1 mL/vial 400μL/vial 1 vial DNase/RNase free H2O

Freeze-dried Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)

Component Character Catalogue Number Component Description
HWTS-RT105A HWTS-RT105B
Specification/Quantity(50 tests/kit) Specification/Quantity(20 tests/kit)
TB Reaction Buffer lyophilized 1 bottle 1 bottle Contains Mycobacterium tuberculosis IS6110 and 38KD, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc.
TB Positive Control lyophilized 1 vial 1 vial Contains IS6110, 38KD and internal control mixed plasmid diluent
TB Blank Control Liquid 1 vial, 600μL/vial 1 vial, 600μL/vial DNase/RNase free H2O
Reconstituted Solution Liquid 1 vial, 1.45mL/vial 1 vial, 1mL/vial DNase/RNase free H2O, etc.

Total PCR Solution

Option 1.

Mycobacterium Tuberculosis DNA Detection Kit7

Option 2.

Mycobacterium Tuberculosis DNA Detection Kit8

Reference

[1]. Programme W. Global tuberculosis control: WHO report [annual].[J]. Who Global Tuberculosis Programme, 2003.
[2]. Kocagoz T, Yilmaz E, Ozkara S, et al. Detection of Mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure.[J]. Journal of clinical microbiology, 1993, 31(6):1435-1438.
[3]. Mikhailovich V, Lapa S, Gryadunov D, et al. Identification of Rifampin-Resistant Mycobacterium tuberculosis Strains by Hybridization, PCR, and Ligase Detection Reaction on Oligonucleotide Microchips[J]. Journal of Clinical Microbiology, 2001, 39(7):2531-2540.
[4]. Miller M B, Popowitch E B, Backlund M G, et al. Performance of Xpert MTB/RIF RUO Assay and IS6110 Real-Time PCR for Mycobacterium tuberculosis Detection in Clinical Samples[J]. Journal of Clinical Microbiology, 2011, 49(10):3458-62.


Product detail pictures:

Quality Inspection for Mycobacterium Tuberculosis Test - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Quality Inspection for Mycobacterium Tuberculosis Test - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Quality Inspection for Mycobacterium Tuberculosis Test - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Quality Inspection for Mycobacterium Tuberculosis Test - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Quality Inspection for Mycobacterium Tuberculosis Test - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures


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    5 Stars By Anastasia from Vancouver - 2018.12.10 19:03
    This company conforms to the market requirement and joins in the market competition by its high quality product, this is an enterprise that have Chinese spirit.
    5 Stars By Claire from South Korea - 2017.08.15 12:36
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