Seven Urogenital Pathogen
Product name
HWTS-UR017A Seven Urogenital Pathogen Nucleic Acid Detection Kit(Melting Curve)
Epidemiology
Sexually transmitted diseases (STDs) are still one of the important threats to global public health security, which can lead to infertility, premature birth, tumors and various serious complications. Common pathogens include chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, herpes simplex virus type 2, ureaplasma parvum, and ureaplasma urealyticum.
Channel
| FAM | CT and NG |
| HEX | MG, MH and HSV2 |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | urethral secretions
Cervical secretions |
| Tt | ≤28 |
| CV | ≤5.0% |
| LoD | CT:500Copies/mL
NG:400Copies/mL MG:1000Copies/mL MH:1000Copies/mL HSV2:400Copies/mL UP:500Copies/mL UU:500Copies/mL |
| Specificity | Test the infected pathogens outside the detection range of the test kit, such as treponema pallidum, candida albicans, trichomonas vaginalis, staphylococcus epidermidis, escherichia coli, gardnerella vaginalis, adenovirus, cytomegalovirus, beta Streptococcus, HIV, lactobacillus casei and human genome. And there is no cross-reactivity.
Anti-interference ability: 0.2 mg/mL bilirubin, cervical mucus, 106 cells/mL white blood cells, 60 mg/mL mucin, whole blood, semen, commonly used antifungal drugs (200 mg/mL levofloxacin, 300 mg/mL erythromycin, 500 mg/mL penicillin, 300mg/mL azithromycin, 10% Jieeryin lotion, 5% Fuyanjie lotion) do not interfere with the kit. |
| Applicable Instruments | SLAN-96P Real-Time PCR Systems
LightCycler®480 Real-Time PCR system |
Work Flow
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
A) Manual method: Take a 1.5mL DNase/RNase-free centrifuge tube and add 200μL of the sample to be tested. The subsequent steps should be extracted in strict accordance with the IFU. The recommended elution volume is 80μL.
B) Automated method: Take the pre-packaged extraction kit, add 200 μL of the sample to be tested into the corresponding well position, and the subsequent steps should be extracted in strict accordance with the IFU.
