Short Lead Time for Gastrointestinal - Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Short Lead Time for Gastrointestinal - Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product Name

Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)

Certificate

CE

Packaging Size

1. Liquid product: 50 tests/kit

2. Freeze-dried product (penicillin vial): 20 tests/kit, 50 tests/kit

3. Freeze-dried product (8-tube strips): 48 tests/kit

Intended Use

This kit is used for in vitro qualitative detection of enterovirus, EV71 and CoxA16 nucleic acids in throat swabs and herpes fluid samples of patients with hand-foot-mouth disease, and provides an auxiliary means for the diagnosis of patients with hand-foot-mouth disease.

Epidemiology

Hand-foot-mouth disease (HFMD) is a common acute infectious disease in children. It mostly occurs in children under 5 years old, and can cause herpes on the hands, feet, mouth and other parts, and a small number of children can cause complications such as myocarditis, pulmonary edema, aseptic meningoencephalitis, etc. Individual children with serious illnesses deteriorate rapidly and are prone to death if not treated promptly.

Currently, 108 serotypes of enteroviruses have been found, which are divided into four groups: A, B, C and D. Enteroviruses that cause HFMD are various, but enterovirus 71 (EV71) and coxsackievirus A16 (CoxA16) are the most common and in addition to HFMD, can cause serious central nervous system complications such as meningitis, encephalitis, and acute flaccid paralysis.

Features

Multiplex PCR Amplification Technology

Internal control: Fully monitor the experimental process to ensure the quality of experiments.

High sensitivity: 500Copies/mL

Wide Coverage Type: Detection of all enteroviruses and phenotyping of EV71 and CoxA16

Channel

FAM: Enterovirus universal RNA

VIC (HEX): CoxA16

ROX: EV71

CY5: Internal control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 15mins No
2 1 cycle 95℃ 10mins No
3 40 cycles 95℃ 15s No
58℃ 30s Yes

 

Technical Parameters

Storage:

Liquid: ≤-18℃ In dark

Lyophilization: ≤30℃

Shelf-life:

Liquid: 9 months

Lyophilization: 12 months

Specimen Type: Throat swab sample, Herpes fluid

Ct: ≤38

CV: ≤5.0%

LoD: 500Copies/mL

 

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.

ABI 7500 Real-Time PCR Systems

ABI 7500 Fast Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

LightCycler®480 Real-Time PCR Systems

LineGene 9600 Plus Real-Time PCR Detection Systems

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Total PCR Solution

● Option 1.

图片3

● Option 2.

图片4

Main Components

Liquid product

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-EV026A EV Reaction Buffer 1.175mL/vial 1 vial Enterovirus universal, EV71,CoxA16 and internal control primers, fluorescent probes, reaction buffer, etc
EV Enzyme Mix 75μL/vial 1 vial Taq enzyme, reverse transcriptase and UDG enzyme, etc.
EV Positive Control 1mL 1 vial Enterovirus universal, EV71, CoxA16 plasmid and internal control plasmid mixture
EV Blank Control 1mL 1 vial DNase/RNase free H2O

 

Lyophilized product

Component Character Catalogue Number Component Description
HWTS-EV020A HWTS-EV020B HWTS-EV020Z
Specification/Quantity(20 tests/kit) Specification/Quantity(50 tests/kit) Specification/Quantity(48 tests/kit)
EV Reaction Buffer lyophilized 1 bottle 1 bottle 8-tube strips, 6 strips Enterovirus universal, EV71, CoxA16 and internal control primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
EV Positive Control lyophilized 1 vial 1 vial 1 vial Enterovirus universal, EV71, CoxA16 plasmid and internal control plasmid mixture
EV Blank Control Liquid 1 vial, 600μL/vial 1 vial, 600μL/vial 1 vial, 600μL/vial DNase/RNase free H2O
Reconstituted Solution Liquid 1 vial, 1mL/vial 1 vial, 1.45mL/vial 1 vial, 1.42mL/vial DNase/RNase free H2O, etc.

Reference

[1] Xiao Xing-Long et al. Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control.[J]. Archives of virology, 2009, 154(1) : 121-5.

[2] Cardosa MJ, Perera D, Brown BA et al (2003) Molecular epidemiology of human enterovirus 71 strains and recent outbreaks in the Asia-Pacific region: comparative analysis of the VP1 and VP4 genes. Emerg Infect Dis 9:461–468.

[3] Oberste MS, Maher K, Flemister MR et al (2000) Comparison of classic and molecular approaches for the identification of untypeable enteroviruses. J Clin Microbiol 38:1170–1174.

[4] Tan EL, Chow VT, Quak SH et al (2008) Development of multiplex real-timehybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16. Diagn Microbiol Infect Dis (in press).

[5] Singh S, Chow VT, Phoon MC, Chan KP, Poh CL. Direct detection of enterovirus 71 (EV71) in clinical specimens from a hand, foot, and mouth disease outbreak in Singapore by reverse transcription-PCR with universal enterovirus and EV71-specific primers. J Clin Microbiol. 2002 Aug;40(8):2823-7.


Product detail pictures:

Short Lead Time for Gastrointestinal - Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Short Lead Time for Gastrointestinal - Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Short Lead Time for Gastrointestinal - Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Short Lead Time for Gastrointestinal - Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Short Lead Time for Gastrointestinal - Enterovirus Universal, EV71 and CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures


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