Six Respiratory Pathogens
Product name
HWTS-RT175-Six Respiratory Pathogens Nucleic Acid Detection Kit (Fluorescence PCR )
Epidemiology
Respiratory infections are the most common group of human diseases that can occur in any gender, age and geographic area and are one of the most significant causes of morbidity and mortality in populations worldwide. The common clinical respiratory pathogens include respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) and Mycoplasma pneumoniae. Clinical signs and symptoms caused by respiratory tract infections are relatively similar, but the treatment, efficacy, and duration of the disease vary among infections caused by different pathogens. Currently, the main methods for laboratory detection of the above respiratory pathogens include: virus isolation, antigen detection and nucleic acid detection. This kit assists in the diagnosis of respiratory viral infections by detecting and identifying specific viral nucleic acids in individuals with signs and symptoms of respiratory infections, in combination with other clinical and laboratory findings.
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab sample |
| Ct | Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| LoD | LoD of Adv, MP, RSV, hMPV, RhV and PIV are all 200Copies/mL |
| Specificity | The results of the cross-reactivity test showed that there is no cross-reactivity between the kit and novel coronavirus, influenza A virus, influenza B virus, human bocavirus, cytomegalovirus, herpes simplex virus type 1, varicella zoster virus, EBV, pertussis bacillus, Chlamydophila pneumoniae, Corynebacterium spp, Escherichia coli, Haemophilus influenzae, Lactobacillus spp, Legionella pneumophila, C. catarrhalis, and attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus salivarius, Actinobacillus baumannii, Narrow-feeding maltophilic monococci, Burkholderia maltophilia, Streptococcus striatus, Nocardia sp., Sarcophaga viscosa, Citrobacter citriodora, Cryptococcus spp, Aspergillus fumigatus, Aspergillus flavus, Pneumatobacteria spp, Candida albicans, Rohypnogonia viscera, oral streptococci, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia q fever and human genomic nucleic acids.
Anti-interfering ability: mucin (60 mg/mL), human blood, benfotiamine (2 mg/mL), oxymetazoline (2 mg/mL), sodium chloride (20 mg/mL), beclomethasone (20 mg/mL), dexamethasone (20 mg/mL), flunitrazolone (20 μg/mL), triamcinolone acetonide (2 mg/mL), budesonide (1 mg/ mL), mometasone (2 mg/mL), fluticasone (2 mg/mL), histamine hydrochloride (5 mg/mL), intranasal live influenza virus vaccine, benzocaine (10%), menthol (10%), zanamivir (20 mg/mL), ribavirin (10 mg/L), paramivir (1 mg/mL), oseltamivir (0.15 mg/mL), mupirocin (20 mg/mL), tobramycin (0.6 mg/mL), UTM, saline, guanidine hydrochloride (5 M/L), Tris (2 M/L), ENTA-2Na (0.6 M/L), trilostane (15%), isopropyl alcohol (20%), and potassium chloride (1 M/L) were subjected to an interference test, the results showed that there was no interference reaction to the detection results of the pathogen at the above concentrations of interfering substances. |
| Applicable Instruments | SLAN-96P Real-Time PCR Systems
Applied Biosystems 7500 Real-Time PCR System QuantStudio®5 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. are recommended for the sample extraction and the subsequent steps should be conducted in strict accordance with the IFU of the Kit.
