Streptococcus Pneumoniae
Product name
HWTS-RT038A/B Streptococcus Pneumoniae Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Respiratory tract infections are divided into upper respiratory tract infections and lower respiratory tract infections, of which lower respiratory tract infections are the most common cause of outpatient visits. Most lower respiratory tract infections are caused by bacterial infections, especially Streptococcus pneumoniae.
Channel
| FAM | Streptococcus Pneumoniae |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Sputum |
| Ct | ≤38 |
| CV | ＜5.0% |
| LoD | 1000 CFU/mL |
| Specificity | There is no cross-reactivity between the kit and other common respiratory pathogens that outside the kit detection range (Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus haemolyticus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella acidophilus, Streptococcus pyogenes, Micrococcus tengchensis, Erysipelas equorum, Listeria griseola, Listeria monocytogenes, Legionella dubliniensis, Enterobacter aerogenes, Haemophilus solubilis, Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus hygroscopicus, Aspergillus fumigatus, Candida glabrata, Candida tropicalis positive samples)Anti-interference ability: 0.2 mg/mL bilirubin, cervical mucus, 106 cells/mL white blood cells, 60 mg/mL mucin, whole blood, semen, commonly used antifungal drugs (200 mg/mL levofloxacin, 300 mg/mL erythromycin, 500 mg/mL penicillin, 300 mg/mL azithromycin, 10% Gelsemide lotion, 5% Gynecological Cleansing Lotions) did not interfere with the test. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Fast Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Recommended extraction reagents: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Add 200 µL of physiological saline to the processed precipitate, and the subsequent steps should be carried out in accordance with the instructions, The recommended elution volume is 100 µL.
