Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test
Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:
Product name
EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin’s disease, T/Natural killer celllymphoma, Burkitt’s lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma[2-4].
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.
Channel
FAM: EBV
VIC (HEX): Internal control
PCR Amplification Conditions Setting
Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
1 | 1 cycle | 50℃ | 2mins | No |
2 | 1 cycle | 95℃ | 5mins | No |
3 | 40 cycles | 95℃ | 15s | No |
58℃ | 30s | Yes |
Technical Parameters
Storage | ≤-18℃ In dark |
Shelf-life | 12 months |
Specimen Type | Whole blood, Plasma, Serum |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 500Copies/mL |
Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
Main Components
Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
HWTS-OT061A | EB Reaction Buffer | 1.175mL/vial | 1 vial | Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc. |
EB Enzyme Mix | 75μL/vial | 1 vial | Taq enzyme, UDG enzyme, etc. | |
EB Positive Control | 600μL/vial | 1 vial | Mixed solution of viral target gene and internal reference | |
EB Blank Control | 600μL/vial | 1 vial | DNase/RNase free H2O |
Total PCR Solution
Reference
[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.
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Gaining client pleasure is our company's aim without end. We are going to make excellent efforts to create new and top-quality goods, meet your special requirements and provide you with pre-sale, on-sale and after-sale companies for Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: San Diego, Ukraine, Gambia, Due to our dedication, our products are well known throughout the world and our export volume continuously grows every year. We will continue to strive for excellence by providing high quality products that will exceed the expectation of our customers.
By Lydia from Colombia - 2017.03.28 12:22
The company account manager has a wealth of industry knowledge and experience, he could provide appropriate program according our needs and speak English fluently.
By Christine from Czech - 2018.11.04 10:32