Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test

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Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)

Certificate

CE

Epidemiology

EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin’s disease, T/Natural killer celllymphoma, Burkitt’s lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma[2-4].

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.

Channel

FAM: EBV
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2mins No
2 1 cycle 95℃ 5mins No
3 40 cycles 95℃ 15s No
58℃ 30s Yes

Technical Parameters

Storage ≤-18℃ In dark
Shelf-life 12 months
Specimen Type Whole blood, Plasma, Serum
Ct ≤38
CV ≤5.0%
LoD 500Copies/mL

Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.

Main Components

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-OT061A EB Reaction Buffer 1.175mL/vial 1 vial Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc.
EB Enzyme Mix 75μL/vial 1 vial Taq enzyme, UDG enzyme, etc.
EB Positive Control 600μL/vial 1 vial Mixed solution of viral target gene and internal reference
EB Blank Control 600μL/vial 1 vial DNase/RNase free H2O

Total PCR Solution

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)6

Reference

[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.


Product detail pictures:

Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Top Suppliers Ebv Pcr Test - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures


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  • This company has the idea of better quality, lower processing costs, prices are more reasonable, so they have competitive product quality and price, that's the main reason we chose to cooperate.
    5 Stars By Elsa from Norway - 2017.07.07 13:00
    The goods are very perfect and the company sales manager is warmful, we will come to this company to purchase next time.
    5 Stars By Cornelia from Lithuania - 2017.06.22 12:49
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