Zika Virus
Product name
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Certificate
CE
Epidemiology
Zika virus belongs to the genus Flaviviridae, is a single-stranded positive-stranded RNA virus with a diameter of 40-70nm. It has an envelope, contains 10794 nucleotides, and encodes 3419 amino acids. According to the genotype, it is divided into African type and Asian type. Zika virus disease is a self-limiting acute infectious disease caused by the Zika virus, which is mainly transmitted through the bite of Aedes aegypti mosquitoes. The clinical features are mainly fever, rash, arthralgia or conjunctivitis, and it is rarely fatal. According to the World Health Organization, neonatal microcephaly and Guillain-Barre syndrome (Guillain-Barré syndrome) may be associated with Zika virus infection.
Channel
| FAM | Zika virus nucleic acid |
| ROX |
Internal Control |
Technical Parameters
| Storage | ≤30℃ & protected from light |
| Shelf-life | 12 months |
| Specimen Type | fresh serum |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Copies/mL |
| Specificity | Use the kit to detect the serum samples with Zika virus negative, and the results are negative. Interference test results show that when the concentration of bilirubin in serum is not more than 168.2μmol/ml, the hemoglobin concentration produced by hemolysis is not more than 130g/L, the blood lipid concentration is not more than 65mmol/ml, the total IgG concentration in serum is not more than 5mg/mL, there is no effect on the dengue virus, Zika virus or chikungunya virus detection. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus and human genomic serum samples are selected for the cross-reactivity test, and the results show that there is no cross reaction between this kit and the pathogens mentioned above. |
| Applicable Instruments | ABI 7500 Real-Time PCR SystemsABI 7500 Fast Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
QIAamp Viral RNA Mini Kit(52904), Nucleic Acid Extraction or Purification Reagent(YDP315-R) by Tiangen Biotech(Beijing) Co.,Ltd. The extraction should be extracted according to the extraction instructions, and the recommended extraction volume is 140 μL and the recommended elution volume is 60 μL.
Option 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). The extraction should be extracted according to the instructions. The extraction sample volume is 200 μL , and the recommended elution volume is 80μL.
